Development of multicolour real time PCR for detection of drug resistance mutation in hepatitis B virus / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To set up a rapid method for detection of drug resistance mutation in HBV, based on multicolour real time PCR. To detect the mutation of blood serum, which were collected from patients.</p><p><b>METHOD</b>To establish two reaction systems, each reaction system contains four resistance loci. On the new multicolor real time PCR method, the sensitivity and specificity were analysed, and detecting the drug resistant mutation of blood serum collected from 30 cases patients.</p><p><b>RESULTS</b>Construction of a multicolor fluorescence PCR for detection drug resistance in HBV was constructed, better specificity, sensitivity analysis of up to 1 x 10(3) copies/ml. Sample of 30 cases were detected, there were 2 YVDD (6.67%), 1 YIDD (3.33%), and there were 5 cases of 1896 variation, accounting for 16.67%. Other sites were not detected mutations.</p><p><b>CONCLUSION</b>The multicolor real time PCR detection system could be used for rapid and simple analysis of drug resistance for the clinical hospital. The 1986 mutation in HBV pre-C region are relatively high.</p>

Experimental study of tissue-engineered biological small intestinal submucosa, membrane employed in dental implant traction / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To construct the tissue-engineered biological small intestinal submucosa(SIS) membrane and evaluate the feasibility of its use as a surrogate of periosteum and its possible role in dental implant distraction (DID).</p><p><b>METHODS</b>The tissue-engineered biological SIS membrane was constructed through the co-culturing of boat bone marrow stromal cells (BMSC) and small intestinal submucosa. The cellular compatibility was evaluated with the phase contrast microscope, SEM, alkaline phosphatase(ALP) activity and histology, and its effect of osteogenesis promotion was detected by Micro-CT and histology after implanted in the exposed side of DID operation.</p><p><b>RESULTS</b>The phase contrast microscope, SEM, ALP activity and histology confirmed that the BMSC could adhere to SIS and proliferate on it normally, the cellular activity and function were not affected by SIS. Three months after the tissue-engineered biological SIS membrane was implanted into the exposed side, some discontinuous new bone in the "biological SIS membrane" group was detected by the Micro-CT under a higher window level. The histology revealed that there was a quantity of new bone in the distracted region and the majority was woven bone. The quantity and quality of the new bone in the "biological SIS membrane" implanted group were similar to the unexposed side, but the bone nonunion was detected in the un-implanted group and the interspace was fixed by fibrous connective tissue.</p><p><b>CONCLUSIONS</b>The tissue-engineered biological SIS membrane can provide both daughter cells, guide bone regeneration in DID and promote the osteogenesis. But the outcome of the biological SIS membrane, especially the function of the osteoblast cultured in the SIS in new bone formation, still needs further study.</p>

Vascularized iliac crest graft with internal oblique muscle for immediate reconstruction of composite mandibular defect / 中华整形外科杂志

<p><b>OBJECTIVE</b>To evaluate the vascularized (deep circumflex iliac vessels) iliac crest graft with internal oblique muscle as a method for reconstruction of composite mandibular defect.</p><p><b>METHODS</b>Vascularized iliac crest graft with internal oblique muscle was used to reconstruct the composite mandibular defects in 10 patients. All clinical data were analyzed retrospectively. A detailed inspection of the case-notes was undertaken to ascertain the presenting diagnosis, the surgery, the complications and the outcome. The type of mandibular defect was recorded.</p><p><b>RESULTS</b>Of the 10 patients with composite mandibular defects including mandibular body, mandibular angle, mandibular ramus and the soft tissue around them, 7 patients were recorded with the defects of mandibular condyles. During the follow-up period from 3 months to 24 months, primary wound healing was observed in all patients, except one patient with minor muscular necrosis. All patients were satisfied with their facial contour and mandibular shape, without tumor recurrence. Donor site problems important enough to be recorded in the notes were minimal.</p><p><b>CONCLUSIONS</b>The vascularized iliac crest graft with internal oblique muscle offers a useful solution for reconstruction of composite mandibular defect. There is sufficient height and depth of bone to maintain a facial contour and mandibular shape. It can be used as a routine surgical technique to reconstruct composite mandibular defect.</p>